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What will you learn on this course?

This one day course is spent learning the theory of liquid chromatography and then using this knowledge to create isocratic and gradient methods, program detectors and analyse data. After an introduction to liquid chromatography, this course addresses solvents & buffers (the mobile phase), pumps, isocratic and gradient elution, sample introduction, analytical columns, HPLC detectors and data analysis.

Learning outcomes

• You will learn the importance of good chromatography
• You will learn about the function & operation of the individual HPLC components
• You will become familiar with the HPLC instrumentation
• You will have practical experience of adjusting the parameters for each HPLC component
• You will have practical experience of analysing data

Prerequisites

This course is suitable for all levels of knowledge; however for complete beginners it is advisable to attend the Absolute Basics of HPLC & LC-MS first to get a good basic overview of liquid chromatography.