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What will you learn on this course?

Module 2 of the Practical Essentials of LC & LC-MS and the Complete LC & LC-MS courses.

This module looks at the types of solvents used in mobile phase and their respective properties are examined in detail. This is followed by information on gradient elution (high or low pressure mixing and solvent dwell times); along with problems that are frequently encountered in this area. Strategies for how to optimise your LC system will be discussed, including the necessity to minimise system void volume to increase performance.

Learning outcomes

You will learn how to select and use the best mobile phase and develop a gradient inlet method.

Prerequisites

If you have limited LC experience, wish to learn more or would like to brush-up on your knowledge about this particular section of the instrument then this is the module for you.